Figure 1From: Beneficial ‘unintended effects’ of a cereal cystatin in transgenic lines of potato, Solanum tuberosumExpression of CCII and potato multicystatin in leaves of transgenic potato lines. A. CCII and ccii transcript levels in leaves of control line K and five independent transgenic lines, as determined by SELDI TOF MS and real-time RT PCR. mRNA transcripts were quantified based on a standard curve with incremental amounts of ccii transgene as a template. CCII levels are expressed as relative amounts compared to line 4.1c (mean value of 1.0). B. Immunodetection of CCII (11.7 kDa) with primary antibodies directed against oryzacystatin I, a structural homologue from rice [22]. Numbers on the right refer to commercial molecular weight markers. PMC refers to endogenous potato multicystatin. C. Relative PMC levels in CCII-lines compared to mean basal level in control line K (arbitrary value of 1). Each bar on panels A and C is the mean of three independent (plant replicate) values ± SE. Asterisks indicate a significant difference with control line K (Student’s t-test; P < 0.05).Back to article page