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Table 3 Novel miRNAs identified in S. rebaudiana

From: smRNAome profiling to identify conserved and novel microRNAs in Stevia rebaudiana Bertoni

miRNA

Sequence

Length

Clones

EST/nt accession no.

Strand

A+U content

Mismatch

miRNA start

miRNA end

EST/nt start

EST/nt end

Precursor

Stv_1

AAACGGUGAACAAGAAGAGG

20

1

BG522782.1

Plus

55%

0

1

20

470

489

YES

Stv_2

UGUGAAGGCGGAUGACUGUAU

21

1

BG523272.1

Plus

55%

0

1

21

216

236

YES

Stv_3

UGAGAUGUUCAAUGGAAAGUAA

22

1

BG526716.1

Plus

68.2%

0

1

22

142

163

YES

Stv_4

GAGACUGAUUUCAGAACCGG

20

1

BG524295.1

Plus

50%

0

1

20

240

259

YES

Stv_5

ACGUACGAUCAUUUGGAUAAGAU

23

1

BG525786.1

Plus

65.2%

0

1

23

167

189

YES

Stv_6

UUGAACAAGUAUGGUCGUCCC

21

5

AY215182.1

Plus

52.3%

0

1

21

435

455

YES

Stv_7

UCGUGCUGUUGGGAAGUGGA

21

1

BG526771.1

Plus

42.3%

0

1

20

327

346

YES

Stv_8

CUGACGUGUCACCAUUACGGA

21

1

BG525715.1

Minus

47.6%

0

1

21

323

303

YES

Stv_9

GGUAAAGCACUGUUUCGGUGC

21

1

BG523023.1

Plus

47.6%

0

1

21

324

344

YES

Stv_10

UUGACACUUUCCCGGGACA

19

2

BG522709.1

Plus

42.1%

0

1

19

95

113

YES

Stv_11

GGCGGGCUCAAAUGACGAAUCAU

23

1

BG524443.1

Plus

47.5%

1

1

23

378

400

YES

Stv_12

CUCGCGCUUUGGUUGAAGAAC

21

1

BG526387.1

Plus

47.6%

0

1

21

266

286

YES

  1. Corresponding hairpin loop structures are provided in supplementary information.