Characterization of the 3-deoxyanthocyanidins in Pr1 and pr1 silks. Compounds were identified based on their retention time to known standards. (A) HPLC chromatograms of silk methanolic extracts at 480 nm. Luteolinidin glycosides a, b, and c were eluted at approximately 13.6 min, 14.1 min, and 14.9 min, respectively. Anthocyanidins elute at approximately 17.9 min. (B) Total luteolinidin and total anthocyanidin levels in silk tissue of homozygous pr1; P1-wr, Pr1; P1-wr, pr1; P1-rr, Pr1; P1-rr, pr1; p-del2, Pr1; p-del2, pr1; P1-ww, and Pr1; P1-ww were determined by HPLC analysis at 495 nm. All data are presented as mean of six replicates.