Subcellular localization of CAN nucleases deletion mutants. ECFP-fusion proteins were transiently expressed in Arabidopsis root cell protoplasts. The bright-field images are shown in the left part of each panel and corresponding confocal laser scanning images are on the right. The images show the cellular location of the ECFP fused proteins lacking the following consensus sequences: (A) CAN2 mutant lacking N-myristoylation and palmitoylation consensus sequences. (B) CAN2 mutant lacking the ABC transporter-like sequence. (C) CAN2 mutant lacking the N-SNc domain. (D) CAN2 mutant lacking the anticodon binding-like sequence (E) CAN2 mutant lacking the C-SNc domain. (F) CAN1 mutant lacking N-myristoylation and palmitoylation consensus sequences. The abbreviations of mutated domains as in Figure 2F The length of the white scale bar at the right bottom of each panel corresponds to 10 μm.