Plasma membrane localization of CAN1-RFP and CAN2-GFP nucleases transiently expressed in Arabidopsis leaf epidermal cells. Confocal images were taken two days after A. tumefaciens infiltration. (A) An epidermal cell expressing CAN1-RFP fusion protein. (B) Higher magnification image of the box in (A). White arrowheads indicate plasma membranes of adjacent cells. (C-E) Epidermal cells expressing the CAN2-GFP fusion protein. (C and E) Higher magnification images of the box in (D) show plasma membranes of adjacent cells before (C) and after (D) plasmolysis. (F) Epidermal cells transformed with empty vector pSITE-2NB-GFP as a control. Strong signal around the nucleus (n) and numerous cytoplasmic strands show characteristic cytoplasmatic localization of GFP protein. The length of the white scale bar at the right bottom of each panel corresponds to 10 μm.