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Table 2 Transmission of p5cs2 mutant alleles in the presence of additional p5cs1-4 mutant alleles

From: Requirement of proline synthesis during Arabidopsis reproductive development

Genotype pollen donor1

Genotype pollen recipient1

Offspring with p5cs2allele [% ±SD2]

Expected frequency3[%]

Seedlings analysed

A/a1 B/B

A/a1 B/B

65.0 ±4.0

66.7

564

A/a1 B/b

A/a1 B/b

52.3 ±3.4*

66.7

521

A/a1 b/b

A/a1 b/b

26.6 ±3.3*

66.7

545

A/A B/B

A/a1 b/b

43.7 ±5.1

50

332

A/A B/B

A/a2 b/b

50.6 ±8.2

50

212

A/A b/b

A/a1 b/b

44.9 ±3.4

50

382

A/A b/b

A/a2 b/b

44.7 ±2.4

50

232

A/a1 b/b

A/A B/B

0.0 ±0.0*

50

166

A/a2 b/b

A/A B/B

1.0 ±1.7*

50

289

A/a1 b/b

A/A b/b

0.0 ±0.0*

50

88

A/a2 b/b

A/A b/b

0.0 ± 0.0*

50

163

  1. Transmission of the p5cs2 mutant alleles was determined by sulfadiazine (p5cs2-1) or BASTA (p5cs2-2) resistance of the progeny after selfing (first three rows) or crossing of various parental genotypes.
  2. 1 A: P5CS2 wildtype allele; a1: p5cs2-1 mutant allele; a2: p5cs2-2 mutant allele; B: P5CS1 wildtype allele; b: p5cs1-4 mutant allele.
  3. 2 values are the mean ±SD of at least 3 independent plants or crossings.
  4. 3 the expected frequency takes into account that homozygous p5cs2/p5cs2 seeds are usually non-viable in the presence of wildtype or heterozygous seeds in the same silique (compare Additional file 2: Figure S2).
  5. * indicates significant difference from the expected value (p<0.03 by Χ2 test with 1 degree of freedom).