Subcellular localization of Sl-ERFs. ERF.B.1, ERF.D.1, ERF.E.1 and ERF.H.1 proteins were fused to the YFP (Yellow Fluorescent Protein) in the N-terminal region and the chimerical proteins were transiently expressed in BY-2 tobacco protoplasts under the control of the 35S promoter. Subcellular localization was then analyzed by confocal laser scanning microscopy. The merged pictures of the yellow fluorescence channel (middle panels) and the corresponding bright field (left panels) are shown (right panels). Control cells expressing YFP alone are shown in the top panel. The scale bar indicates 10 μm.