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Figure 2 | BMC Plant Biology

Figure 2

From: Characterization of a calcium/calmodulin-regulated SR/CAMTA gene family during tomato fruit development and ripening

Figure 2

SlSRs are calcium/calmodulin-binding proteins. (A) Alignment of the conserved putative calmodulin-binding regions of SlSRs with AtSRs. The conserved amino acids are highlighted with either green (hydrophobic residues) or purple (positively charged residues). (B) Helical wheel projection showing the basic amphipathic α-helix structure in the predicted calcium/calmodulin-binding domain of SlSR3. (C) Calmodulin-binding overlay assay showing that calmodulin binds SlSR3 only in the presence of calcium. Total proteins from E. coli carrying pETSlSR3 were analyzed using SDS-PAGE, transferred onto a PVDF membrane and incubated with biotinylated calmodulin. 1, No IPTG induction; 2, IPTG added. (D) Gel mobility shift assay showing that calcium loaded calmodulin binds to the synthetic peptide corresponding to the putative calmodulin-binding region in SlSR3 (peptide amino acid sequence shown at the top of panel D). Arrows indicate the positions of free calmodulin (CaM) and the peptide-calmodulin complex.

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