Characterisation of transgenic tobacco plants. (a) Schematic representation of the pCAMBIA2301 - 35S: SbSOS1 construct used to transform tobacco plants with the SbSOS1 gene. (b, c) Verification of transgenic lines via PCR analysis of the SbSOS1 gene with real-time PCR primers and gus-specific primers in T0 plants. (d) Leaf disc assay of WT and transgenic lines (L1 and L7) at different NaCl concentrations in T0 plants. (e) The graph represents the mean and standard deviation (SD) of chlorophyll content in leaf discs of WT and transgenic lines (L1 and L7) at different NaCl concentrations. (f) GUS assay of T1 plants showing positive GUS expression in the transgenic line and negative expression in WT plants. (g) Transcript levels of the SbSOS1 gene in transgenic lines (L1, L7 and L8) and WT T1 plants via semiquantitative RT-PCR.