Transient p35S-eGFP-LjNIP1 expression in Nicotiana tabacum epidermal leaf cells. A-C. Free-eGFP fluorescence is homogenously present inside the epidermal cells and is accumulated above all in the plasma membrane and inside the nucleus (n), whereas the red chlorophyll autofluorescence is limited to the chloroplasts. D. A light green signal, due to eGFP-LjNIP1 expression, is accumulated in the inner membrane system (ms), starting from the nuclear envelop (ne). A weak signal is also associated to the plasma membrane. E. Chlorophyll fluorescence of the same leaf shown in D. F. Overlay image of D and E. Bars = 25 μm.