Water permeability of LjNIP1 and LjXIP1. Water permeability of intact yeast protoplasts expressing LjNIP1 (blue), LjXIP1 (dark green), K10 (red) and the empty vector pDR199 (maroon). Yeast swelling kinetics were recorded as time courses of the decreased scattered light intensity in a stopped flow spectrophotometer. The differences of scattered light are correlated with the capacity of protoplasts to acquire water in the rapid mixing device. LjNIP1 resulted to be able of transferring water whereas LjXIP1 did not show any difference with the empty vector pDR199.