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Table 4 Stability of selected housekeeping genes in the developing barley grain

From: Quantitative RT-PCR based platform for rapid quantification of the transcripts of highly homologous multigene families and their members during grain development

DAP

Actin/Ubiquitin

Actin/PTF

Ubiquitin/Actin

Ubiquitin/PTF

PTF/Actin

PTF/Ubiquitin

10

0.16 ± 0.01

0.12 ± 0.01

6.15 ± 0.19

0.72 ± 0.02

8.56 ± 0.33

1.39 ± 0.05

15

0.13 ± 0.03

0.17 ± 0.04

7.53 ± 0.48

1.26 ± 0.08

6.57 ± 0.23

0.79 ± 0.14

18

0.14 ± 0.03

0.20 ± 0.04

6.17 ± 0.93

1.42 ± 0.34

5.99 ± 0.24

0.71 ± 0.24

25

0.11 ± 0.00

0.08 ± 0.00

9.21 ± 0.32

0.70 ± 0.02

13.19 ± 0.38

1.44 ± 0.04

  1. Ratio: difference in the amount of the two housekeeping genes; calculated as amol x 10-3.
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BMC Plant Biology

ISSN: 1471-2229

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