Target validation of conserved Oryza sativa miRNAs. Experimental validation of miRNA-mRNA cleavage site was performed using a modified 5′RACE assay. Each miRNA is shown base-paired to its predicted target mRNA. The sequences of miRNAs are shown in 3′ to 5′ direction. The two points indicate a Watson-Crick base-pair, one point indicates a Wooble base-pair and the space indicates a mismatch. The arrow above the target mRNA indicates the 5′ end of cleavage products. Three different experiments were performed for each combination.