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Table 1 Distribution of the G. soja sequences in the Al-treated and Al-free libraries

From: Identification of wild soybean miRNAs and their target genes responsive to aluminum stress

RNA class Al-treated Al-free
  Counts Percentage of total Counts Percentage of total
Raw reads 8,616,284 100.00% 8,712,410 100.00%
Number of reads removed due to 3ADTa not found 974,498 11.31% 1,411,300 16.20%
Number of reads removed due to <15 bases after 3ADT cut 345,537 4.01% 260,266 2.99%
Junk reads 8,594 0.10% 4,930 0.06%
Number of mapped reads 7,287,655 84.58% 7,035,914 80.76%
mRNAb 6,310,043 86.59% 6,114,595 86.91%
Rfamc 192,054 2.64% 164,494 2.34%
Repeatsd 2,976 0.04% 2,140 0.03%
Known and predicted miRNAs 184,734 2.53% 124,343 1.77%
No hit 597,848 8.20% 630,342 8.96%
  1. a, 3ADT is the 3’ adaptor.
  2. b, The G. max mRNAs were downloaded from ftp://ftp.jgi-psf.org/pub/JGI_data/phytozome/v7.0/Gmax/annotation/Gmax_109_transcript.fa.gz[56].
  3. c, Rfam (V 10.0) was downloaded from ftp://ftp.sanger.ac.uk/pub/databases/Rfam/9.1/[54].
  4. d, Repbase (V13.12) was downloaded from http://www.girinst.org/repbase/update/index.html[55].