Skip to main content
Figure 4 | BMC Plant Biology

Figure 4

From: Identification of defensin-encoding genes of Picea glauca: characterization of PgD5, a conserved spruce defensin with strong antifungal activity

Figure 4

Purification of recombinant PgD5 produced by E. coli BL21 (Origami pLys S) DE3. (A) RP-HPLC chromatography of the His6-SUMO-PgD5 fusion protein after cleavage with the SUMO protease. Elution times are marked. (B) SDS-PAGE analysis of the His6-SUMO-PgD5 fusion before and after SUMO protease treatment. Lane M, low molecular weight marker (New England Biolabs). Lane 1, Purified fusion protein by Ni-NTA column. Lane 2, SUMO protease cleavage products after 60 min. Lane 3, Purified PgD5 peptide after SUMO protease digestion and reverse-phase chromatography. (C) Mass spectrometric analysis of recombinant PgD5 after separation from the His6-SUMO tag using reverse-phase chromatography.

Back to article page

BMC Plant Biology

ISSN: 1471-2229

Contact us