Figure 3

Transcriptional activation by RGL2 involves promoter motifs bound by Dof-type transcription factors. Mesophyll protoplasts were transfected with reporter constructs carrying different promoter motifs 5’ of a minimal promoter (m35S) to drive GFP expression (B). Transfected protoplasts were treated with MOCK, DEX, or DEX plus GA₃, and GFP signal was analysed by confocal microscopy (A). Signal intensities of 6 to 10 protoplasts of each construct/treatment-combination were determined using the Carl Zeiss LSM software, and plotted relative to the signal obtained from GFP driven by m35S only (C).