Figure 5

Phenotypes induced by a dominant negative FIL:SRDX construct. (A,B) 35S _pro ^I>>FIL plants grown on media without DEX (A) or with DEX (B). (C,D) 35S _pro ^I>>FIL:SRDX plants grown on media without DEX (C) or with DEX (D). (E) A fil yab3/FIL _pro ::FIL plant displaying full complementation. Inset: fil yab3 double mutant plant. (F) fil yab3/FIL _pro ::FIL:SRDX seedlings showing narrow cotyledons that are sometimes bifurcated (asterisk). Cotyledons of fil yab3 (G) and fil yab3 yab5 mutant seedlings (H). (I) fil yab3/FIL _pro ::FIL:SRDX plant with needle-like leaves. (J) fil yab3 yab5 triple mutant plant with narrow and needle-like leaves. (K) Histochemical staining for YAB3:GUS activity in a fil yab3/FIL _pro ::FIL:SRDX plant. YAB3 promoter activity is detected throughout young radial leaves. Inset: fil yab3/FIL _pro ::FIL stained for GUS activity. (L) A fil/+ yab3/FIL _pro ::FIL:SRDX plant with a fil yab3 mutant leaf phenotype. (M-R) Scanning electron micrograph showing the abaxial epidermis of wildtype (M), fil yab3 (N), fil yab3 FIL _pro ::FIL (O) and fil/+ yab3/FIL _pro ::FIL:SRDX (P) leaves. Note that the larger cell morphology in (N,P) is due to leaf adaxialisation. (Q,R) SEM showing epidermal cell morphology of fil yab3 yab5 needle leaves (Q) and those of fil yab3/FIL _pro ::FIL:SRDX plants (R). Scale bars are 5 mm in (E,L) and inset in (A); 2 mm for (A-D, F-H); 1 mm for (I,J); 200 μm for (K) and inset in (K); 100 μm in (M-R).