Table 5 Primer sequences used for the PCR analysis of putative transgenic regenerants
Primer | Sequence 5′ – 3′ |
|---|---|
35S-F2-C | CATGGTGGAGCACGACACTCTC |
35S-term-C | CATGAGCGAAACCCTATAAGAACCC |
GH-35S-term-F1 | AATCACCAGTCTCTCTCTAC |
GH-Hyg-F1 | GATCGGACGATTGCGTCGCA |
GH-Hyg-R2 | TATCGGCACTTTGCATCGGC |
GH-Ubi-F3 | CCGTTCCGCAGACGGGATCGATCTAGGATAGGTA |
GH-GFP-F1 | GGTCACGAACTCCAGCAGGA |
GH-GFP-R2 | TACGGCAAGCTGACCCTGAA |