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Table 4 The integrity, functionality and segregation of the T-DNA copies present in four primary transgenics

From: Analysis of T-DNA integration and generative segregation in transgenic winter triticale (x TriticosecaleWittmack)

T0 T1 T2
T-DNA PCR DNA gel blot Phenotype Phenotype
  P35S-HPT HPT-T35S PUBI1-gfp gfp-TNOS HPT gfp gfp segregation integration loci hygromycinR (leaf assay) GFP (seedlings)
TG5E01
copy #1 + + + + + + 14:6 (2.3:1) 3 + + b
copy #2 - - n.d. a + + + 18:2 (9.0:1)   -  
copy #3 - - + - - + 17:3 (5.6:1)   - +
TG5E02
copy #1 + + + + + + 18:2 (9.0:1) 1 + +
TG5E03
copy #1 + b + b + b + b + + 3:17 (0.2:1) 1c + b + b
copy #2      + +     
TG5E04
copy #1 - + - + + + 11:8 (1.4:1) 2 - -
copy #2 + + + + + + 12:7 (1.7:1)   + +
  1. a) Not determined, because an analysis independent of any of the two other copies was impossible.
  2. b) Positive for at least one of the two transgene copies, however, these copies could not be analysed independent of one another.
  3. c) Single case of this study where two T-DNA copies were co-integrated in one chromosomal locus.