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Table 4 The integrity, functionality and segregation of the T-DNA copies present in four primary transgenics

From: Analysis of T-DNA integration and generative segregation in transgenic winter triticale (x TriticosecaleWittmack)

T0

T1

T2

T-DNA

PCR

DNA gel blot

Phenotype

Phenotype

 

P35S-HPT

HPT-T35S

PUBI1-gfp

gfp-TNOS

HPT

gfp

gfp segregation

integration loci

hygromycinR (leaf assay)

GFP (seedlings)

TG5E01

copy #1

+

+

+

+

+

+

14:6 (2.3:1)

3

+

+ b

copy #2

-

-

n.d. a

+

+

+

18:2 (9.0:1)

 

-

 

copy #3

-

-

+

-

-

+

17:3 (5.6:1)

 

-

+

TG5E02

copy #1

+

+

+

+

+

+

18:2 (9.0:1)

1

+

+

TG5E03

copy #1

+ b

+ b

+ b

+ b

+

+

3:17 (0.2:1)

1c

+ b

+ b

copy #2

    

+

+

    

TG5E04

copy #1

-

+

-

+

+

+

11:8 (1.4:1)

2

-

-

copy #2

+

+

+

+

+

+

12:7 (1.7:1)

 

+

+

  1. a) Not determined, because an analysis independent of any of the two other copies was impossible.
  2. b) Positive for at least one of the two transgene copies, however, these copies could not be analysed independent of one another.
  3. c) Single case of this study where two T-DNA copies were co-integrated in one chromosomal locus.