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Table 3 Transgene segregation in twelve T 2 populations, based on gfp expression in seedling tissue

From: Analysis of T-DNA integration and generative segregation in transgenic winter triticale (x TriticosecaleWittmack)

T1 plants T-DNA copies (DNA gel blot) T2 plants analyzed Segregation of gfp expression Segregation ratio observed/ (presumed for hemizygosity) Zygosity concluded
TG5E01-6 1 (#3) 25 25:0 25:0 (3:1) homozygous
TG5E01-12 2 (#1 + #3) 22 18:4 4.5:1 (15:1) hemizygous
TG5E01-19 2 (#2 + #3) 25 25:0 25:0 (15:1) homozygousn.s.
TG5E01-20 2 (#1 + #2) 28 28:0 28:0 (15:1) homozygous n.s.
TG5E02-2 1 22 22:0 22:0 (3:1) homozygous *
TG5E02-4 1 17 17:0 17:0 (3:1) homozygous*
TG5E02-9 1 20 20:0 20:0 (3:1) homozygous*
TG5E03-1 2 (#1 + #2) 21 7:14 1:2 (3:1) hemizygous
TG5E03-7 2 (#1 + #2) 20 4:16 1:4 (3:1) hemizygous
TG5E03-8 0 20 0:20 0:20 azygous
TG5E04-2 2 (#1 + #2) 18 14:4 3.5:1 (15:1) hemizygous
TG5E04-3 1 (#1) 17 0:17 0:17 (3:1) unascertainable1
TG5E04-12 0 14 0:14 0:14 azygous
  1. *) Significant, n.s.: not significant, according to χ2 test.
  2. 1) TG5E04-3 carries a non-functional copy of gfp.