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Table 1 Effect of pre-cultivation, A. tumefaciens strain and co-cultivation medium on the generation of stable transgenic triticale

From: Analysis of T-DNA integration and generative segregation in transgenic winter triticale (x TriticosecaleWittmack)

Strain Pre-culture time and co-culture conditions Transgenic lines per 100 IEs
AGL-1/ pYF133 0 d, BCCM -
0 d, WCCM   -
  5 d, BCCM -
  5 d, WCCM -
  2 d, BCCM, filter paper -
  5 d, BCCM, filter paper -
  5 d, WCCM, filter paper -
LBA4404/ pSB187 0 d, BCCM -
0 d, WCCM   -
  5 d, BCCM -
  5 d, WCCM -
  2 d, BCCM, filter paper 3.6
  5 d, BCCM, filter paper 4.0
  5 d, WCCM, filter paper -
  1. A set of 50 IEs was processed per treatment. BCCM - barley co-culture medium, WCCM - wheat co-culture medium [13], IEs - immature embryos.