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Figure 7 | BMC Plant Biology

Figure 7

From: Arabidopsis mutant sk156 reveals complex regulation of SPL15 in a miR156-controlled gene network

Figure 7

Leaf primodium-dependent miR156-insensitive SPL15m restores WT phenotypes when expressed in sk156 . The sk156 background was transformed with native miR156-sensitive SPL15n or miR156-insensitive SPL15m expression cassettes each controlled by either an AS1 or 35S promoter. (A) SPL15 transcript levels by qPCR (± standard deviation) in transgenic sk156 plants complemented with AS1:SPL15m, AS1:SPL15n, 35S:SPL15n or SPL15:SPL15m compared to WT (set at 1) and sk156 (p < 0.05). (B) and (C) Morphology at bolting (32 d) and maturity (50 d) for plant expressing SPL15n or SPL15m under the control of AS1 promoter compared to sk156. (D) Morphology of 42 d flowering lines for sk156 plant carrying 35S:SPL15n or SPL15:SPL15m compared to sk156. (E) and (F) Carotenoid levels (± standard error) in seeds of sk156 complemented with AS1:SPL15m, AS1:SPL15n , 35S:SPL15n or SPL15:SPL15m compared to those of WT and sk156. Panels A, E, F: A Duncan's multiple range test was conducted to separate significantly different means for more than 10 independent transgenic lines (each measured with triplicated seed batches) relative to WT plants at p < 0.05 (*).

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