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Table 3 Relative transcript abundance of sucrolytic and related regulatory genes

From: Metabolic and enzymatic changes associated with carbon mobilization, utilization and replenishment triggered in grain amaranth (Amaranthus cruentus) in response to partial defoliation by mechanical injury or insect herbivory

Gene 1

Treatment

dppd

Leaf

Stem

Root

Panicle

AhSuS-1

HD

1

0.18 ± 0.00

1.50 ± 0.20

2.09 ± 0.10

—

5

1.38 ± 0.05

0.87 ± 0.21

0.83 ± 0.08

—

30

3.01 ± 0.24

2.15 ± 0.40

1.03 ± 0.19

2.15 ± 0.08

MD

1

0.63 ± 0.06

NE

0.95 ± 0.16

—

5

0.87 ± 0.07

0.84 ± 0.07

0.53 ± 0.08

—

30

1.23 ± 0.27

1.06 ± 0.30

0.55 ± 0.01

1.47 ± 0.35

AhSuS-2

HD

1

0.36 ± 0.03

2.10 ± 0.10

1.64 ± 0.21

—

5

1.21 ± 0.02

0.77 ± 0.09

1.52 ± 0.03

—

30

2.62 ± 0.07

1.58 ± 0.14

1.77 ± 0.25

1.91 ± 0.05

MD

1

0.76 ± 0.04

2.36 ± 0.41

1.19 ± 0.10

—

5

0.86 ± 0.08

0.66 ± 0.01

0.86 ± 0.23

—

30

1.13 ± 0.08

2.00 ± 0.21

1.10 ± 0.06

1.07 ± 0.04

AhVI-1

HD

1

2.19 ± 0.18

3.00 ± 0.90

1.37 ± 0.13

—

5

3.74 ± 0.00

6.58 ± 0.26

2.49 ± 0.05

—

30

0.91 ± 0.25

1.12 ± 0.15

1.20 ± 0.18

1.38 ±0.02

MD

1

0.82 ± 0.01

1.25 ± 0.06

1.96 ± 0.14

—

5

2.57 ± 0.06

2.71 ± 0.76

1.30 ± 0.43

—

30

1.24 ± 0.04

1.11 ± 0.13

0.77 ± 0.06

1.65 ± 0.04

AhInvI-4 (VI inhibitor)

HD

1

0.40 ± 0.17

1.00 ± 0.30

1.54 ± 0.11

—

5

1.00 ± 0.01

0.89 ± 0.34

0.78 ± 0.22

—

30

1.77 ± 0.29

0.60 ± 0.06

0.72 ± 0.18

1.30 ± 0.22

MD

1

0.68 ± 0.40

1.12 ± 0.39

0.86 ± 0.09

—

5

0.96 ± 0.06

0.61 ± 0.34

0.61 ± 0.16

—

30

2.89 ± 0.53

1.86 ± 0.04

0.98 ± 0.10

0.76 ± 0.09

Ah-γVPE

HD

1

4.40 ± 0.54

0.70 ± 0.10

1.41 ± 0.19

—

5

0.10 ± 0.01

1.91 ± 0.25

2.74 ± 0.52

—

30

5.83 ± 0.92

0.98 ± 0.30

1.61 ± 0.13

2.09 ± 0.11

MD

1

0.49 ± 0.20

0.59 ± 0.27

1.15 ± 0.11

—

5

0.35 ± 0.04

0.39 ± 0.12

0.13 ± 0.01

—

30

3.59 ± 0.01

1.10 ± 0.55

1.39 ± 0.14

1.76 ± 0.09

AhInvI-2 (CWI inhibitor)

HD

1

0.23 ± 0.02

1.40 ± 0.20

1.51 ± 0.23

—

5

1.59 ± 0.14

0.85 ± 0.06

0.75 ± 0.10

—

30

1.94 ± 0.28

1.12 ± 0.02

0.97 ± 0.03

1.39 ± 0.16

MD

1

0.35 ± 0.19

2.45 ± 1.69

1.29 ± 0.16

—

5

1.30 ± 0.00

0.56 ± 0.04

1.00 ± 0.06

—

30

0.88 ± 0.01

2.56 ± 0.15

0.94 ± 0.16

1.26 ± 0.10

AhInvI-1 (CWI inhibitor)

HD

1

0.20 ± 0.03

2NE

0.29 ± 0.06

—

5

0.53 ± 0.09

0.96 ± 0.01

0.98 ± 0.00

 

30

1.03 ± 0.13

0.91 ± 0.16

0.70 ± 0.03

1.05 ± 0.13

MD

1

0.70 ± 0.05

NE

1.22 ± 0.16

—

5

0.63 ± 0.04

1.19 ± 0.51

1.86 ± 0.54

—

30

0.94 ± 0.22

2.48 ± 0.23

2.08 ± 0.19

0.93 ± 0.15

AhA/NI-1

HD

1

0.09 ± 0.20

0.79 ± 0.26

NE

—

  

5

0.94 ± 0.37

3.59 ± 0.31

NE

—

  

30

NE

1.38 ± 0.16

NE

NE

 

MD

1

0.11 ± 0.20

1.64 ± 0.06

NE

—

  

5

0.22 ± 0.47

6.10 ± 0.22

NE

—

  

30

NE

0.80 ± 0.17

NE

NE

AhSNRK-1

HD

1

1.87 ± 0.05

—

—

—

5

1.29 ± 0.38

—

—

—

30

0.40 ± 0.27

—

—

—

MD

1

0.18 ± 0.16

—

—

—

5

4.70 ± 0.50

—

—

—

  

30

3.87 ± 0.27

—

—

—

  1. Expression was measured at different days post partial defoliation (dppd) by insect (HD) or mechanical (MD) damage in different plant tissues of Amaranthus cruentus. Transcript levels were measured by qRT-PCR. Values represent means ± SE of a representative experiment with three replicates. They indicate the fold-change in gene expression levels present in tissues of defoliated plants relative to those in tissues of intact control plants. A given gene was considered induced when its relative expression values was ≥ 1.5, whereas it was considered repressed when its relative expression values was ≤ 0.5. These are shown in bold text and bold italics, respectively.
  2. 1The expression of the neutral invertase AhA/NI-2 and sucrose phosphate synthase (AhSPS) genes was not detected in any of the tissues examined.
  3. 2NE = Not Expressed.
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BMC Plant Biology

ISSN: 1471-2229

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