Table 5 Primers used in this study
Primer name | Sequence 5’ → 3’ |
|---|---|
RPS10B genomic construct | |
RPS10Bgenomic-F | AAACTAGTAACCGAGTAAACGGGATGATTAGG |
RPS10Bgenomic-R | AAAAAAAGCTTAGCTCCTCAACATTCAACTCCTTC |
RPS10B promoter::RPS10A, RPS10B and RPS10C cDNA constructs | |
RPS10Bpro-F | GGATCC GCGGCCGCTGAATAAGTAACATCAAACTCCAGCTA |
RPS10Bpro-R | ATCATGATTGCGATGAGATTGAAGAAGGA |
RPS10AcDNA-F | CTTCAATCTCATCGCAATCATGATTATCTCAGAGAACAATCGCAG |
RPS10AcDNA-R | AATCTAGATCAAGGGAACCCTGAACCAGATGGTGCT |
RPS10BcDNA-F | CTTCAATCTCATCGCAATCATGATCATATCAGAGACTAACCGCCGT |
RPS10BcDNA-R | AATCTAGA TCAAGGAAGATCAGATCCAGCAGCA |
RPS10CcDNA-F | CTTCAATCTCATCGCAATCATGATTATCTCAGAGGCTAACCGCAAA |
RPS10CcDNA-R | AATCTAGATCAAGGCAAACCTGAACCAGATGGTGCA |
RT-PCR | |
RPS10A-RT-F | AGATTTGGTGACCGTGATGGATAC |
RPS10A-RT-R | CCTTCCATCGTCGCAATATGAC |
RPS10B-RT-F | AGGTTTGGTGACAGAGATGGATAC |
RPS10B-RT-R | AGACCAAAAAGAAACAAGAAAGTCC |
RPS10C-RT-F | GTTTGGTGACCGTGATGGGTAC |
RPS10C-RT-R | AACTCCTCCATGGTCTTACTGTC |
CCD7-F | CCGAGTCAAGCTTAATCCAATAG |
CCD7-R | ATTGCAGTTTCCGGTAGAGTCCAA |
CCD8-F | CATCGGCGATCAACAAATAA |
CCD8-R | GTTTAACCAAATCCGGTATC |
Ubiquitin5-F | AACCCTTGAGGTTGAATCATC |
Ubiquitin5-R | GTCCTTCTTTCTGGTAAACGT |
Actin2-RT-F | TTACCCGATGGGCAAGTCA |
Actin2-AT-Rev | CACCACTGAGCACAATGTTAC |