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Figure 6 | BMC Plant Biology

Figure 6

From: Mutation of the cytosolic ribosomal protein-encoding RPS10Bgene affects shoot meristematic function in Arabidopsis

Figure 6

Dosage effects of REV and PID in the rps10b-1 mutant background. (a-g) F2 from a cross of rps10b-1 with the rev mutant. (a-d) Rosettes of F2 plants genotyped for RPS10B and REV. The primary inflorescences were removed. White arrows mark axillary shoots (branches or axillary buds). In the presence of functional RPS10B (one copy in b, two copies in c), loss of one (b) or both (c) functional REV copies does not noticeably affect rosette branching. In the rps10b mutant background (a,d) loss of one functional REV copy (d) completely abolishes axillary bud formation. (e) Quantification of axillary shoot development of genotyped F2 segregants. The proportions of rosette, cauline and floral nodes showing normal versus abnormal axillary shoot development are plotted. For vegetative nodes, development was classified as abnormal if the axil appeared empty. For floral nodes, development was classified abnormal when the node was occupied by a pedicel- or filament-like structure, and normal when it carried a flower or silique. For the rosette nodes of rev and rps10b/+ rev/+ plants only the number of branches was scored, thus the white bar represents a minimum estimate of the proportion of normal nodes and the proportion of abnormal nodes is not given. 7 to 21 individuals were scored per genotypic class. (f,g) Some leaves of rps10b rev/+ plants had outgrowths from the midvein at the abaxial side. rps10b/+ rev/+ leaves appeared normal. (h-k) F3 from a cross of rps10b-1 with the pid-14 T-DNA insertion allele (SALK_049736). A rps10b pid-14/+ segregant with two basal nodes lacking axillary shoots (i, white arrows), occurrence of this phenotype in pid-14/+ controls (h) was negligible and in rps10b controls (j) less frequent (see k). (k) Quantification of cauline and rosette axillary shoot development of genotyped F3 segregants. Analysis was done as in (e). 7 to 16 individuals were scored per genotypic class.

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