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Table 1 Substrate specificity of SlGOMT1 and SlGOMT2 with various substrates

From: Identification of white campion (Silene latifolia) guaiacol O-methyltransferase involved in the biosynthesis of veratrole, a key volatile for pollinator attraction

Substrate

SlGOMT1* (% Activity)

SlGOMT2 (% Activity)

Guaiacol

100

12

Catechol

35

13

Orcinol

11

9

Eugenol

<1

<1

Methyleugenol

<1

<1

  1. Purified SlGOMT1 and SlGOMT2 were assayed at room temperature under standard assay conditions by adding substrate at a final concentration of 200 μM and excess 14C-SAM together. Relative activity is expressed as the percentage of GOMT1 activity with guaiacol as a substrate.
  2. * Specific activity was measured 1.96 NMOL/MIN/MG protein at saturating substrate concentrations.
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BMC Plant Biology

ISSN: 1471-2229

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