DNA binding specificity of ATAF2, a NAC domain transcription factor targeted for degradation by Tobacco mosaic virus

Table 2 Gene expression analysis on ATAF2 candidate target genes

Clone number	AGI	Annotation	Wt Col. 5 hr wounded vs. non-wounded*	ATAF2 KO-136355 5 hr wounded vs. non-wounded*
	AT5G08790	ATAF2	5.37	1
C7	AT1G08540	RNA Polymerase sigma subunit	1.42	0.47
C32	AT2G28390	SAND family protein	1.64	0.91
C34	AT1G68907	Defensin-like (DEFL) family protein	3.63	0.99
C52	AT3G26540	Pentatricopeptide (PPR) repeat-containing protein	2.00	0.78
C104	AT3G62060	Pectinacetylesterase family protein	2.91	1.06
C108	AT4G19570	DNAJ heat shock N-terminal domain-containing protein	8.06	2.17
C113	AT3G11700	Fasciclin-like arabinogalactan protein 18 precursor, FLA18	3.69	1.02
C116	AT5G56550	Oxidative stress 3, OXS3	3.68	1.55
C123	AT3G59050	Polyamine oxidase 3, AtPAO3	14.98	5.92

* qRT-PCR analysis of ATAF2 and selected candidate ATAF2 target genes at 5 hrs post leaf wounding vs non-wounded tissues. Fold changes are the average of two independent experiments. For each experiment leaf RNA was pooled from 5 individual test plants and qRT-PCR reactions were run in duplicate and normalized to 18 s rRNA levels.
Wild-type Columbia and ATAF2 knock-out (Salk_136355) plants were mechanically wounded 5 hr prior to RNA extraction.

ISSN: 1471-2229