Figure 7From: DNA binding specificity of ATAF2, a NAC domain transcription factor targeted for degradation by Tobacco mosaic virusScanning mutagenesis analysis of the ATAF2 binding sequence. (A) Six different two base substitutions (M1 – M6) were created within the 30-bp binding sequence. Specific mutations are underlined. (B) EMSA assays testing ATAF2-binding activity. The binding activity was expressed relative to ATAF2 binding to wild-type 30-bp sequence as 100%.Back to article page