Figure 6

Mutations within the 30-bp sequence affect ATAF2-DNA binding activity. (A) Schematic diagram of mutants created within the 30-bp ATAF2 binding sequence. (B) EMSA assay showing all three mutations have reduced ATAF2 binding activity. (C) Both GUS staining assay and GUS quantification assay confirming that all three mutants affected ATAF2-DNA binding activity in vivo. Tissues punches from three independent leaves were collected at two days post agro-infiltraton and tested by fluorometric assay for GUS activity. Data represents the average ± standard deviation.