Figure 5

TMV infection enhances GUS activity driven by the ATAF2 binding sequence. (A) Real-time qRT-PCR analysis showing increased ATAF2 expression in Agro-infiltrated and TMV-inoculated tissues at 6 dpi. Numbers 0.1 and 0.5 are the mg/ml of virus used for inoculation. RNA was extracted from three independent test plants. Data represents average ± standard deviation from triplicate qRT-PCR reactions. (B) TMV coat protein specific Western-blot showing the level of virus accumulation in three independent samples of TMV-inoculated tissues. (C) Enhanced GUS activity observed in TMV-inoculated tissues. At four dpi TMV-infected Arabidopsis plants were agro-infiltrated with the 35S minimal promoter (35SM-GUS), the 2X30bp-GUS, or the 4X30bp-GUS reporter constructs. At six dpi tissue punches from three independent leaves were collected and tested by fluorometric assay for GUS activity. Data represents the average ± standard deviation.