Figure 5

In situ localization of EgPG4 transcripts in the fruit base containing the AZ prior to cell separation. (A-C) Longitudinal sections of the fruit base were hybridized with digoxigenin-labelled antisense RNA fragments of EgPG4 and (D) the 18S ribosome and expression is observed as a blue colouring using bright field microscopy. Sections were made from fruit prior to ethylene treatment (A, E, H) and after 6 h of ethylene treatments (B-D, F, G, I, J). (E-G) Sections were also observed using polarized light and (H-J) epifluorescence microscopy to distinguish the AZ from the adjacent pedicel (P) and mesocarp (M) tissues.