Skip to main content

Table 2 Phosphorylated proteins stained by Pro-Q Diamond and identified by MALDI-TOF/TOF-MS during grain filling

From: Proteome characterization of developing grains in bread wheat cultivars (Triticum aestivum L.)

Spot no.

Accession no.

Protein name

Protein score

C.I.%

Total Ion score

Total Ion C.I.%

Theoretical pI/MW(kDa)

Experimental pI/MW(kDa)

Matched sequences

Predicted number of phosphorylation sitesa

Ser

Thr

Tyr

67*

gi/171027826

Triticin

100

84

100

6.43/64.90

8.79/30.52

2

18

2

4

70*

gi/215398470

Globulin 3

100

471

100

7.78/66.31

7.58/63.57

6

17

6

2

94*

gi/38098487

Alpha amylase inhibitor protein

100

152

100

7.44/18.21

6.10/16.70

3

2

1

0

96*

gi/54778501

0.19 dimeric alpha-amylase inhibitor

100

360

100

6.66/13.33

6.29/16.79

4

   

100*

gi/225042

Alpha amylase inhibitor

100

259

100

6.77/19.62

6.87/20.32

4

8

2

3

102*

gi/54778507

0.19 dimeric alpha-amylase inhibitor

100

373

100

5.73/13.24

6.07/15.90

4

2

0

2

112*

gi/62465514

Class II chitinase

100

95

100

8.66/28.20

8.81/30.60

2

9

3

1

113*

gi/62465514

Class II chitinase

100

69

99.38

8.66/28.20

9.23/30.23

1

9

3

1

114*

gi/62465514

Class II chitinase

100

138

100

8.66/28.20

8.52/30.56

2

9

3

1

122*

gi/22001285

Peroxidase 1

100

138

100

8.14/38.80

8.55/51.53

4

11

5

2

123*

gi/22001285

Peroxidase 1

100

206

100

8.14/38.80

8.22/51.66

4

11

5

2

a#

gi/4558484

Heat shock protein 101

100

288

100

5.95/101.06

5.20/90.65

6

21

3

7

b#

gi/4204859

Heat shock protein 80

100

286

100

5.00/80.33

3.29/83.11

5

24

9

6

c#

gi/18146829

Chitinase 3

100

63

100

6.89/33.46

8.42/31.12

1

10

6

4

d#

gi/34925030

RecName: Full = Wheatwin-1; AltName:Full = Pathogenesis-related protein 4a

100

275

100

7.57/15.62

5.97/15.54

4

2

2

1

e#

gi/34925032

RecName: Full = Wheatwin-2; AltName:Full = Pathogenesis-related protein 4b

100

224

100

8.18/15.86

6.88/15.63

4

2

2

1

  1. aNumber of phosphorylation sites predicted by NetPhos 2.0 Server.
  2. *Spot numbers correspond to those shown in Figure 2 and Figure 3.
  3. #Newly identified phosphorylated protein spots.