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Table 1 Differentially expressed proteins identified by MALDI-TOF/TOF-MS at five grain developmental stages in bread wheat cultivars Jimai 20 and Zhoumai 16

From: Proteome characterization of developing grains in bread wheat cultivars (Triticum aestivum L.)

Spot no.a

Accession no.b

Protein name

Species

Protein score C.I.%c

Total Ion score

MPd

EpI/TpIe

Average volume ratio (Jimai 20)

Average volume ratio (Zhoumai 16)

HCP (J/Z)f

Predicted subcellular Localizationg

I:II:III:IV:V

p-value

I:II:III:IV:V

p-value

Carbohydrate metabolism

1. TCA pathway:

22

gi/49343245

Cytosolic malate dehydrogenase

T. aestivum

100

161

6

5.36/5.75

1:1.1:2.4:1.3:0.3

0.015

1:1.2:0.8:0.3:0.2

0.036

C/C

Cyto

23

gi/49343245

Cytosolic malate dehydrogenase

T. aestivum

100

51

2

5.76/5.75

1:1.5:2.6:1.4:0.4

0.023

1:1.4:1.0:0.4:0.3

0.030

C/C

Cyto

24

gi/37928995

Cytosolic malate dehydrogenase

T. aestivum

100

266

3

5.98/6.62

1:0.7:.0.4:0.3:0

0.038

1:0.7:0.6:0:0

0.027

A/A

Cyto

25

gi/15232820

MDH (malate dehydrogenase)

A. thaliana

100

101

2

6.82/8.66

1:0.8:0.8:0.5:0.3

0.019

1:0.7:1.0:0.7:0.4

0.019

A/A

P

32

gi/7488742

Isocitrate dehydrogenase (NADP) precursor

M. sativa

99

144

3

5.54/6.15

1:0.6:0.7:0.5:0.1

0.041

1:0.9:0.5:0.4:0.2

0.011

A/A

P

33

gi/92875135

Isocitrate dehydrogenase NADP-dependent

M. truncatula

97.38

98

2

6.02/5.99

1:0.8:0.5:0.3:0.3

0.021

1:0.5:0.5:0.2:0.2

0.018

A/A

Mito

2. Glycolysis:

1

gi/32400802

Phosphoglycerate mutase

T. aestivum

100

71

2

5.55/5.43

1:0.4:0.4:0.2:0.1

0.032

1:0.5:0.3:0.2:0

0.029

A/A

Cyto

2

gi/32400802

Phosphoglycerate mutase

T. aestivum

100

55

2

5.45/5.43

1:0.7:0.5:0.3:0.2

0.017

1:0.6:0.6:0.5:0.3

0.034

A/A

Cyto

4

gi/120680

Glyceraldehyde-3-phosphate dehydrogenase, cytosolic

H. vulgare

100

323

4

6.46/6.67

1:1.4:0.9:0.4:0.2

0.025

1:1.0:0.3:0.3:0.1

0.015

C/A

Cyto

5

gi/120680

Glyceraldehyde-3-phosphate dehydrogenase, cytosolic

H. vulgare

100

430

4

6.67/6.67

1:1.2:0.9:0.5:0.4

0.014

1:1.5:0.8:0.5:1.1

0.014

C/C

Cyto

6

gi/120668

Glyceraldehyde-3-phosphate dehydrogenase, cytosolic

H. vulgare

100

317

4

6.43/6.20

1:2.1:0.5:0.8:0.9

0.024

1:1.9:1.0:0.5:0.6

0.022

C/C

Cyto

7

gi/148508784

Glyceraldehyde-3-phosphate dehydrogenase

T. aestivum

100

99

4

6.44/7.08

1:1.6:0.6:0.5:0.2

0.026

1:1.2:0.4:0.4:0.1

0.011

C/C

Cyto

8

gi/148508784

Glyceraldehyde-3-phosphate dehydrogenase

T. aestivum

100

137

4

7.47/7.08

1:1.3:0.4:0.4:0.3

0.031

-

-

C/-

Cyto

9

gi/148508784

Glyceraldehyde-3-phosphate dehydrogenase

T. aestivum

100

126

2

7.36/7.08

1:1.4:3.2:5.8:0.2

0.037

1:1.3:6.2:3.2:2.4

0.030

C/C

Cyto

11

gi/18978

Glyceraldehyde 3-phosphate dehydrogenase

H. vulgare

99.83

351

4

6.82/6.67

1:1.3:1:0.6:0.2

0.011

1:0.9:1.7:1.1:0.1

0.010

C/C

Cyto

12

gi/18978

Glyceraldehyde 3-phosphate dehydrogenase

H. vulgare

100

131

1

7.23/6.67

1:0.8:0.5:0.2:0

0.029

1:0.5:0.2:0:0

0.024

A/A

Cyto

13

gi/7579064

Cytosolic glyceraldehyde-3- phosphate

dehydrogenase GAPDH

T. aestivum

100

238

3

6.16/7.83

0:0:0:1:1.3

0.041

0:0:0:1.0:1.9

0.011

B/B

Cyto

14

gi/32478662

Cytosolic glyceraldehyde-3- phosphate

dehydrogenase

T. aestivum

100

208

2

6.66/6.34

1:1.2:0.8:0.6:0.3

0.005

1:1.4:0.8:0.5:0.5

0.023

C/C

Cyto

16

gi/28172909

Cytosolic 3-phosphoglycerate kinase

T. aestivum

100

52

2

4.76/4.91

1:1.6:2.1:1.7:0.4

0.019

1:1.9:1.6:2.0:0.6

0.035

C/C

Cyto

18

gi/18076790

Phosphoglucomutase (PGM)

T. aestivum

100

73

2

5.84/5.66

1:1.3:2.3:0.9:0.8

0.017

1:1.7:3.9:0.6:0.4

0.029

C/C

Cyto

19

gi/18076790

Phosphoglucomutase (PGM)

T. aestivum

98.67

47

1

5.85/5.66

1:0.7:0.2:0:0

0.026

1:0.5:0.2:0:0

0.015

A/A

Cyto

20

gi/226316441

Fructose-bisphosphate aldolase

T. aestivum

100

64

2

6.33/6.85

0:0:1.0:3.1:3.0

0.023

0:0:1.0:4.1:4.7

0.016

B/B

Cyto

21

gi/18496065

Putative fructose 1-,6-biphosphate aldolase

T. aestivum

98.12

84

1

6.15/8.71

1:0.5:0.2:0:0

0.034

1:0.4:0.2:0:0

0.034

A/A

Cyto

26

gi/1785948

Cytosolic triosephosphate isomerase

H. vulgare

100

148

2

5.18/5.39

1:1.1:0.7:0.5:1.4

0.036

1:2.1:1.6:2.0:1.7

0.025

E/E

Cyto

27

gi/11124572

Triosephosphat-isomerase

T. aestivum

100

116

2

4.46/5.38

1:1.4:0.6:0:0

0.013

1:0.8:0.4:0:0

0.022

C/A

Cyto

29

gi/11124572

Triosephosphat-isomerase

T. aestivum

100

235

3

5.72/5.38

1:1.4:0.8:0.5:0.2

0.012

1:0.9:0.5:0.3:0

0.037

C/A

Cyto

37

gi/133872550

Bp2A protein

T. aestivum

100

132

3

5.38/5.86

1:1.5:1:0.7:0.2

0.022

1:0.8:0.6:0.5:0

0.033

C/A

Cyto

3. Alcoholic fermentation:

38

gi/32400847

Formate dehydrogenase

T. aestivum

100

68

2

6.02/8.61

0:0:1.0:2.6:2.9

0.033

0:0:0:1.0:1.5

0.019

B/B

Cyto

39

gi/32400847

Formate dehydrogenase

T. aestivum

100

112

3

6.45/8.61

0:0:1.0:3.4:6.1

0.031

0:0:1.0:3.2:7.8

0.028

B/B

Cyto

4. Starch metabolism:

35

gi/21680

ADP-glucose pyrophosophorylase preprotein

T. aestivum

95.78

54

2

6.35/6.61

1:0.8:0.7:0.6:0.4

0.027

1:0.8:0.6:0.6:0.3

0.027

A/A

P

41

Q0PG36

Glucose-1-phosphate adenylyltransferase

T. aestivum

100

183

5

6.02/6.12

1:1.3:0.4:0.2:0.2

0.029

1:1.2:0.4:0.2:0.1

0.034

C/C

P

44

Q0PG36

Glucose-1-phosphate adenylyltransferase

T. aestivum

100

110

3

6.42/6.12

1:0.9:0.6:0.4:0.2

0.021

1:1.5:0.8:0.4:0.3

0.024

A/C

P

45

Q0PG36

Glucose-1-phosphate adenylyltransferase

T. aestivum

100

125

3

6.23/5.89

1:0.8:0.5:0.2:0.1

0.037

1:1.3:0.6:0.3:0.2

0.016

A/C

P

5. Lipid and sterol metabolism:

30

gi/167113

Aldose reductase-related protein

B. inermis

98.12

101

1

6.19/6.28

0:0:0:1.0:1.8

0.034

0:0:0:1.0:6.8

0.025

B/B

Cyto

31

gi/167113

Aldose reductase-related protein

B. inermis

99.83

116

2

6.17/6.28

0:0:0:1.0:0.8

0.036

0:0:1.0:1.8:1.5

0.028

C/C

Cyto

40

gi/218157

Cytoplasmic aldolase

O. sativajaponica

100

110

2

6.65/6.56

1:0.6:0.3:0.2:0.1

0.031

1:0.9:0.4:0.3:0.1

0.019

A/A

Cyto

145

gi/66840998

5a2 protein

T. aestivum

100

187

4

5.84/8.38

1:8.3:3.5:2.1:2.4

0.038

1:1.1:0.5:0.7:0.7

0.024

C/C

P

Protein synthesis/assembly/degradation

36

gi/41052632

Peptidylprolyl isomerase Cyp2

O. sativajaponica

96.38

50

2

8.97/8.61

1:0.2:0.8:0.9:1.1

0.012

1:0.5:0.5:0.4:0.8

0.014

D/D

Cyto

54

gi/154761388

Cyclophilin

T. aestivum

98.21

19

2

8.39/8.59

1:0.3:1.3:1.4:1.6

0.027

1:0.5:0.4:0.4:0.8

0.011

D/D

Cyto

55

gi/154761388

Cyclophilin

T. aestivum

100

84

3

9.12/8.59

1:0.2:0.8:0.9:1.1

0.031

1:0.6:0.5:0.5:0.9

0.009

D/D

Cyto

56

gi/154761388

Cyclophilin

T. aestivum

100

95

3

6.28/8.59

0:0:0:1.0:6.8

0.013

-

-

B/-

Cyto

59

gi/32352154

Nascent polypeptide associated complex alpha chain

O. sativajaponica

100

83

1

4.17/4.34

0:0:0:1.0:2.1

0.016

0:0:0:1.0:3.0

0.028

B/B

Cyto

62

gi/2492077

Sequence 5 from patent US 5668007

T. aestivum

100

189

2

6.68/6.77

0:1.0:3.5:2.1:5.3

0.027

0:1.0:0.9:3.0:6.7

0.017

B/B

Cyto

106

gi/75279909

Serpin-Z2B; AltName: Full = TriaeZ2b;

T. aestivum

100

213

4

5.24/5.18

0:0:0:1.0:0.6

0.021

0:0:1.0:1.7:0.5

0.016

C/C

P

107

gi/75279909

Serpin-Z2B; AltName: Full = TriaeZ2b;

T. aestivum

100

256

4

5.32/5.18

1:1.8:2.4:1.4:0.9

0.023

0:0:1.0:1.7:1.4

0.021

C/C

P

128

gi/12229936

Proteasome subunit alpha type 7 (20S proteasome alpha subunit D)

C. arietinum

100

110

2

12.10/6.86

1:1.1:5.1:5.9:8.3

0.032

1:1.3:3.2:3.9:6.3

0.033

B/B

P

Storage proteins

67

gi/171027826

Triticin

T. aestivum

100

84

2

8.94/6.43

0:0:1.0:1.4:3.0

0.042

0:0:1.0:2.0:3.3

0.045

B/B

ER

68

gi/171027826

Triticin

T. aestivum

100

475

6

6.35/6.43

0:0:1.0:2.1:5.5

0.039

0:0:1.0:2.4:6.5

0.041

B/B

ER

69

gi/7548844

Triticin precursor

T. aestivum

99.92

169

3

6.05/9.37

1:0.8:0.5:0.6:0.4

0.026

1:0.4:0.5:0.4:0.3

0.038

A/A

ER

70

gi/215398470

Globulin 3

T. aestivum

100

471

5

7.24/7.78

0:0:0:1.0:2.6

0.019

0:0:0:1.0:2.0

0.008

B/B

ER

71

gi/215398470

Globulin 3

T. aestivum

100

148

5

7.43/7.78

0:0:0:1.0:6.1

0.025

0:0:0:1.0:3.9

0.010

B/B

ER

72

gi/89143122

Putative avenin-like b precursor

T. aestivum

100

166

3

6.88/8.08

0:0:1.0:3.3:2.1

0.010

1.0:1.7:6.5:2.9:5.

0.034

C/C

ER

73

gi/145321072

Avenin-like protein

T. aestivum

100

97

2

6.63/8.29

0:0:1.0:3.2:4.1

0.024

0:0:1.0:3.5:4.0

0.031

B/B

ER

74

gi/209971847

Gamma-gliadin

T. aestivum

99.92

70

1

6.84/7.55

0:0:1.0:2.0:4.8

0.029

1.0:1.7:6.5:9.4:12.8

0.028

B/B

ER

75

gi/133741924

Gamma gliadin

T. aestivum

100

93

2

5.33/8.88

1:2.5:4.2:9.9:11.9

0.020

1:2.2:3.9:5.9:6.4

0.022

B/B

ER

76

gi/133741924

Gamma gliadin

T. aestivum

98.12

52

2

5.56/8.88

0:1:2.9:3.8:4.7

0.034

0:0:1.0:1.4:2.2

0.036

B/B

ER

77

gi/209971907

Gamma-gliadin

T. aestivum

97.35

55

2

8.55/8.64

0:1:2.0:2.2:4.2

0.031

0:0:1.0:1.6:2.1

0.031

B/B

ER

78

gi/164470668

LMW-s glutenin subunit 0359D24-S

T. aestivum

99.83

60

2

8.40/8.48

0:0:1:3.6:5.0

0.027

0:0:0:1.0:1.2

0.030

B/B

ER

79

gi/47607146

S-type low molecular weight glutenin L4-55

T. aestivum

100

90

2

8.67/8.51

0:0:0:1.0:2.9

0.024

-

-

B/-

Cyto

80

gi/215398468

Globulin 3C

T. aestivum

100

80

2

6.37/9.15

0:0:0:1.0:1.6

0.026

0:0:0:1.0:2.0

0.041

B/B

ER

81

gi/421978

Globulin Beg1 precursor

H. vulgare

100

85

2

5.64/6.81

0:0:0:1.0:4.2

0.031

0:0:0:1.0:3.0

0.044

B/B

ER

82

gi/167004

Embryo globulin

H. vulgare subsp. vulgare

95.67

53

1

6.79/6.81

0:0:0:1.0:4.4

0.011

0:0:0:1.0:2.5

0.025

B/B

ER

84

gi/170696

Storage protein

T. aestivum

100

52

1

9.49/6.82

0:0:1.0:1.4:1.7

0.012

0:0:1.0:0.8:2.0

0.018

B/B

ER

Nitrogen metabolism

47

gi/164471780

Aspartate aminotransferase

T. aestivum

99.96

25

2

6.61/6.77

1:1.3:1.0:0.6:0.1

0.019

1:0.9:0.8:0.6:0.2

0.027

C/A

Cyto

48

gi/164471780

Aspartate aminotransferase

T. aestivum

100

25

2

6.95/6.77

1:0.8:0.5:0.2:0.3

0.021

1:0.6:0.5:0.2:0.2

0.024

A/A

Cyto

49

gi/584706

Aspartate aminotransferase, cytoplasmic

O. sativajaponica

100

159

3

6.89/7.75

-

-

1:0.7:0.5:0:0

0.031

-/A

Cyto

51

gi/14018051

Putative alanine aminotransferase

O. sativajaponica

100

107

3

6.11/6.23

1:2.4:3.3:2.6:1.5

0.034

1:3.4:2.1:2.1:1.1

0.034

C/C

Cyto

52

gi/56315117

Predicted serine-pyruvate aminotransferase

O. sativajaponica

100

113

1

6.18/5.86

0:0:0:1.0:2.7

0.031

0:0:0:1.0:1.7

0.018

B/B

Cyto

53

gi/6006863

Putative methionine synthase

A. thaliana

98.76

69

1

5.88/6.09

1:2.4:3.3:2.6:1.5

0.030

1:0.7:0.5:0:0

0.016

C/A

Cyto

Transcription/translation

60

gi/479830

Translation elongation factor eEF-1 beta' chain

T. aestivum

100

64

1

4.63/4.54

0:0:0:1.0:2.2

0.016

0:0:0:1.0:1.7

0.028

B/B

Cyto

85

gi/18419557

Transposase

T. aestivum

97.54

98

4

6.53/9.48

1:1.8:6.1:1.6:0.2

0.015

1:1.5:2.2:1.7:1.1

0.027

C/C

Nucl

86

gi/30793446

27 K protein

T. aestivum

98.12

71

1

6.01/6.06

-

-

1:1.9:2.6:2.7:3.3

0.035

-/B

Cyto

87

gi/30793446

27 K protein

T. aestivum

95.89

69

1

5.98/6.06

0:0:0:1.0:2.1

0.045

0:0:0:1.0:2.3

0.031

B/B

Cyto

88

gi/30793446

27 K protein

T. aestivum

100

171

4

5.64/6.06

1:1.9:2.0:3.6:3.4

0.025

1:1.1:2.1:1.6:2.0

0.012

B/E

Cyto

89

gi/46394372

TPA: TPA_inf: WRKY transcription factor 59

O. sativa

95.45

113

2

5.46/7.7

1:1.4:2.2:3.0:2.9

0.018

1:1.8:2.2:4.4:2.7

0.028

B/C

P

90

gi/30793446

27 K protein

T. aestivum

100

60

1

6.06/6.06

0:0:0:1.0:2.3

0.021

0:0:0:1.0:2.2

0.015

B/B

Cyto

91

gi/27735373

Replication factor C like protein

T. aestivum

100

64

1

5.84/8.92

1:0.5:0.3:0.2:0.2

0.026

1:1.4:0.8:0.7:0.5

0.024

A/C

Nucl

93

Q03033

Elongation factor 1-alpha

T. aestivum

100

97

2

9.35/9.20

0:0:0:1.0:2.1

0.034

0:0:0:1.0:2.6

0.026

B/B

Nucl

Signal transduction

64

gi/134290443

Pm3b-like disease r esistance protein 15Q1

T. aestivum

96.58

96

2

7.21/6.27

1:1.3:0.40.4:0.3

0.029

1:1.0:0.4:0.3:0.4

0.024

C/A

Cyto

65

gi/18145

Putative protein has homology to G protein

beta subunit

C. reinhardtii

100

60

1

5.89/7.59

0:1.0:3.2:1.6:0

0.042

1:1.0:1.6:1.2:0

0.026

C/C

Cyto

66

gi/50932677

Putative guanine nucleotide- binding protein

beta subunit

O. sativa

100

121

2

6.07/6.06

0:1.0:2.7:1.4:0

0.044

1:1.1:3.4:2.2:0.8

0.034

C/C

Cyto

142

gi/9652119

Nucleoside diphosphate kinase

L. perenne

99.92

192

2

6.36/6.30

1:0.9:0.7:0.4:0.2

0.019

1:0.8:0.8:0.5:0.2

0.021

A/A

Cyto

Stress/defense

57

gi/640015

CMx

T. aestivum

100

102

2

5.51/9.23

0:0:1.0:2.5:1.8

0.036

0:0:1.0:2.8:2.4

0.022

C/C

P

58

gi/75107149

RecName: Full = Chymotrypsin inhibitor WCI; AltName: Full = Chloroform/ methanol-soluble

protein WCI

T. aestivum

100

164

3

7.34/7.42

0:0:1.0:3.3:1.9

0.027

0:0:1.0:2.7:0.9

0.015

C/C

Cyto

94

gi/38098487

Alpha amylase inhibitor protein

T. aestivum

99.91

62

2

6.65/7.44

0:0:1.0:3.4:4.0

0.021

0:0:1.0:3.0:3.5

0.018

B/B

P

95

gi/66841026

Alpha-amylase inhibitor 0.19

T. aestivum

100

94

2

7.72/6.86

0:0:1.0:2.0:1.2

0.031

0:0:1.0:2.7:2.0

0.024

C/C

P

96

gi/66841026

Alpha-amylase inhibitor 0.19

T. aestivum

100

493

5

6.98/6.86

0:0:1.0:4.3:5.0

0.021

0:1.0:1.7:3.9:2.7

0.038

B/C

P

97

gi/123956

Alpha-amylase/trypsin inhibitor CM2;

T. aestivum

100

191

2

5.71/6.86

0:0:1.0:2.2:1.2

0.018

0:1.0:1.4:3.5:1.1

0.016

C/C

Extr

98

gi/221855644

Alpha-amylase inhibitor CM16 subunit

T. macha

99.96

60

2

4.56/5.31

0:0:1.0:4.2:1.4

0.021

0:0:1.0:2.3:1.7

0.035

C/C

Extr

99

gi/221855644

Alpha-amylase inhibitor CM16 subunit

T. macha

100

97

2

5.11/5.31

0:0:1.0:3.1:1.6

0.024

0:1.0:2.5:5.4:3.2

0.026

C/C

Extr

100

gi/225042

Alpha amylase inhibitor

T. aestivum

100

64

2

6.65/6.77

1:2.7:9.4:5.5:13.9

0.029

1:4.1:10.4:6.1:13.1

0.036

E/E

Cyto

101

gi/123955

Alpha-amylase/trypsin i nhibitor CM1;

T. aestivum

100

73

2

6.81/7.49

0:0:1.0:3.1:3.0

0.012

1:1.0:1.4:4.1:3.3

0.012

B/C

Extr

102

gi/54778507

0.19 dimeric alpha-amylase inhibitor

T. aestivum

100

238

2

6.76/5.73

1:1.5:2.6:2.4:2.3

0.025

1:1.6:2.1:2.7:2.4

0.028

C/C

P

104

gi/408873

Puroindoline = basic cystine-rich protein

T. aestivum

100

125

4

9.05/5.60

1:1.5:2.8:2.9:2.6

0.016

1:1.2:1.7:4.0:1.4

0.023

C/C

P

105

gi/408873

Puroindoline = basic cystine-rich protein

T. aestivum

100

125

4

6.73/8.76

0:0:1.0:3.0:5.2

0.039

0:0:1.0:2.0:2.5

0.045

B/B

P

110

gi/51247633

Chain A, Crystal Structure Of Family 11

Xylanase In Complex With Inhibitor (Xip-I)

T. aestivum

100

78

2

8.24/5.18

0:0:1.0:0.9:3.1

0.038

0:0:1.0:3.2:7.2

0.008

B/B

P

111

gi/51247633

Chain A, Crystal Structure Of Family 11

Xylanase In Complex With Inhibitor (Xip-I)

T. aestivum

100

163

3

8.32/5.18

0:0:1.0:1.4:1.5

0.027

0:0:1.0:4.5:5.8

0.015

B/B

P

112

gi/62465514

Class II chitinase

T. aestivum

100

95

2

8.39/5.56

0:0:1.0:1.1:1.8

0.025

0:0:1.0:0.7:1.7

0.028

B/B

Extr

113

gi/62465514

Class II chitinase

T. aestivum

100

54

1

8.42/8.27

0:0:1.0:1.1:1.6

0.024

0:0:1.0:1.2:2.0

0.015

B/B

Extr

114

gi/62465514

Class II chitinase

T. aestivum

100

138

2

8.37/8.27

1:2.5:5.8:3.0:9.8

0.025

1:1.2:2.1:2.1:6.2

0.025

B/B

Extr

115

gi/62465514

Class II chitinase

T. aestivum

100

69

3

8.35/8.27

0:0:1.0:1.2:1.9

0.038

0:0:1.0:0.8:3.9

0.005

B/B

Extr

116

gi/62465514

Class II chitinase

T. aestivum

100

206

4

7.40/8.66

0:0:1.0:1.4:1.5

0.027

0:0:1.0:1.6:2.1

0.034

B/B

Extr

117

gi/25989705

LEA1 protein

T. aestivum

100

117

2

9.21/8.81

0:0:0:1.0:2.9

0.012

0:0:0:1.0:2.7

0.021

B/B

Nucl

119

gi/134034615

Monomeric alpha-amylase inhibitor

T. aestivum

99.98

45

2

5.54/5.37

0:0:0:1.0:2.0

0.019

0:0:0:1.0:1.6

0.025

B/B

P

120

gi/134034615

Monomeric alpha-amylase inhibitor

T. aestivum

100

47

2

5.65/5.37

-

-

0:1.0:1.6:2.1:2.7

0.015

-/B

P

121

gi/22001285

Peroxidase 1

T. aestivum

99.99

58

3

7.35/8.14

1:1.3:0.4:0.4:0.3

0.038

1:1.0:0.4:0.1:0.4

0.045

C/A

Extr

122

gi/22001285

Peroxidase 1

T. aestivum

100

266

4

8.39/8.14

0:1.0:3.0:3.9:3.9

0.031

0:0:1.0:0.6:1.3

0.004

B/B

Extr

123

gi/22001285

Peroxidase 1

T. aestivum

100

136

6

8.42/8.14

0:0:1.0:0.6:0.8

0.029

0:0:1.0:0.8:1.6

0.026

C/B

Extr

124

gi/75246527

Translationally-controlled tumor protein

T. aestivum

100

203

3

4.24/4.55

1:1.5:0.7:0.3:0.2

0.025

1:2.2:1.4:1.1:0.6

0.049

C/C

Cyto

125

gi/20257409

Thaumatin-like protein

T. aestivum

100

255

6

7.65/7.85

0:0:0:1.0:6.7

0.024

0:0:1.0:2.3:2.0

0.030

B/B

P

126

gi/20257409

Thaumatin-like protein

T. aestivum

100

162

2

7.80/7.85

0:0:0:1.0:8.9

0.016

0:0:1.0:1.3:0.9

0.034

B/C

P

127

gi/226897529

Superoxide dismutase

T. aestivum

100

72

2

5.97/5.71

-

-

1.0:2.1:1.9:4.2:3.2

0.015

-/C

Cyto

Photosynthesis

132

gi/11990897

Ribulose-1,5-bisphosphate carboxylase

/oxygenase small subunit

T. aestivum

100

209

7

6.20/8.80

1.0:1.2:0.4:0.4:0.3

0.049

1:1.1:0.7:0.6:0.2

0.034

A/C

P

140

gi/34393258

Putative Oxygen-evolving enhancer protein 3–1,

chloroplast precursor

O. sativajaponica

99.83

59

1

9.45/9.82

1:0.6:0.5:0.4:0.4

0.018

1:0.7:0.6:0.4:0.4

0.031

A/A

P

Others

143

gi/357152329

Probable beta-D-xylosidase 7-like

T. aestivum

100

47

2

7.89/7.53

0:0:1.0:0.5:0.5

0.042

0:0:1.0:0.5:0.5

0.049

C/C

P

146

gi/2454602

Barperm1

H. vulgare subsp. vulgare

100

207

4

8.09/8.15

0:0:1.0:1.6:0.4

0.018

0:0:1.0:1.6:0.5

0.045

C/C

P

147

gi/115458852

Os04g0465600

O. sativajaponica

100

92

1

7.41/4.75

0:0:1.0:1.1:1.8

0.021

0:0:1.0:1.1:1.8

0.025

B/B

Cyto

  1. aSpot no: corresponds to protein spot on gels shown in Figure 2 and Figure 3.
  2. bAccession no: predicted protein in NCBInr and UniprotKB database.
  3. cProtein Score C.I.%: the PMF score percentage of protein sequence (Confidence interval: Protein Score C.I.% ≥ 95%).
  4. dMP: Matched peptides.
  5. eEpI/TpI: pI of protein on the gel/pI of predicted protein.
  6. fHCP(J/Z): hierachical cluster pattern A-E (Jimai 20/Zhoumai 16).
  7. gCyto: cytosol; P: plastid; Mito: mitochondria; ER: endoplasmic reticulum; Nucl: nuclear; Extr: extracellular.
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BMC Plant Biology

ISSN: 1471-2229

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