Protoplast transient expression analyses using HAT-YFP fusion constructs. Subcellular localization of OsHAC701, OsHAG702, and OsHAG704 was determined via Arabidopsis protoplast PEG transfection using HAT-YFP fusion constructs. OsHAC701 and OsHAG702 were localized in both the nucleus and cytosol. OsHAG704 was localized in the nucleus and cytosol, although YFP signals were relatively weak. Red color indicated autofluorescence emitted by chloroplasts. VirD2NLS-mCherry was used as a nuclear marker. Scale bars = 7.5 μm.