Figure 1
Characterisation of WRKY transcription factors from various species. (A) Identical amino acids are highlighted in blue. The approximately 60-amino acid WRKY domain and the C and H residues in the zinc-finger motif (C-X4-5-C-X22-23-H-X1-H) are marked by the two-headed arrow and triangle, respectively. The short conserved HARF structural motif and the highly conserved amino acid sequence WRKYGQK in the WRKY domain are boxed. (B) Phylogenetic analysis of GhWRKY15 in relation to other plant WRKY transcription factors. The WRKY transcription factors used are as follows: GhWRKY15 (GU207867) and GhWRKY3 (ADO51775) from G. hirsutum, PtWRKY26 (ACV92028), PtWRKY24 (ACV92026), PtWRKY12 (ACV92014) and PtWRKY21 (ACV92023) from P. tomentosa, StWRKY2 (ABU49721) from S. tuberosum, AtWRKY15 (NP_179913), AtWRKY7 (NP_194155), AtWRKY11 (NP_567878), AtWRKY4 (NP_172849), AtWRKY40 (NP_178199), AtWRKY31 (NM_118328), AtWRKY6 (NM_104910), AtWRKY28 (NM_117927), AtWRKY48 (NM_124329), AtWRKY14 (NM_102802), AtWRKY22 (NM_116355), and AtWRKY41 (XP_00287254) from A. thaliana, BnWRKY7 (ACQ76809) from Brassi canapus, CaWRKY2 (ABA56495) and CaWRKY30 (ACJ04728) from Capsicum annuum, HvWRKY10 (ABI13376) from Hordeum vulgare, VvWRKY2 (XP_002264243) from Vitis vinifera, OsWRKY71(AY676927) and OsWRKY1 (AAF23898) from O. sativa and NcWRKY53(ABN79278) from Noccaea caerulescens.