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Figure 1 | BMC Plant Biology

Figure 1

From: Loss of compatibility might explain resistance of the Arabidopsis thaliana accession Te-0 to Golovinomyces cichoracearum

Figure 1

PTI and ETI markers in G. cichoracearum- infected Col-0 , Te-0, and Kas-1 plants. (a) ROS accumulation revealed by the presence of brown-reddish precipitates in tissues treated with 3,3 diaminobenzidine (DAB). Fungal structures are stained with trypan blue. Haustoria (arrows) and attempted fungal penetration sites (arrowheads) are indicated. Bars: 30 μm. (b) Callose deposition evaluated by confocal microscopy of aniline blue stained leaves. Callose accumulates at the cell wall of Kas-1 epidermal cells contacting conidia (C; arrowheads). In Col-0 and Te-0 tissues, fluorescence is detected around the haustoria (HA; arrows), but not at the cell wall of penetrated cells. Fungal autofluorescence is also depicted. H: hyphae. Bars: 30 μm. (c) Plant cell death, evidenced by trypan blue staining, is only found in Kas-1 tissues (arrowhead). Haustorial-like structures are shown by dashed lines. Fungal structures are stained with trypan blue. Bars: 30 μm. Insets on (a) and (c) correspond to 4X enlargements of the shown pictures.

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