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Figure 2 | BMC Plant Biology

Figure 2

From: Virus-induced gene-silencing in wheat spikes and grains and its application in functional analysis of HMW-GS-encoding genes

Figure 2

Silencing endogenous phytoene desaturase gene ( PDS ) in wheat spikes. Wheat cv. Xiaoyan 6 was infected with in vitro transcribed RNAs representing the α, β and γ RNAs of BSMV:00 or α, β and γPDS RNAs of BSMV:PDS onto the spikes at the heading stage. (A) Left photograph indicates the phenotypes of wheat plants respectively infected with BSMV:00 (1) and BSMV:PDS (2) at 22 days post-inoculation (dpi); the right one is the spikes photographed at 13dpi, 22dpi and 30dpi, respectively. The spikes shown are representative of different treated plants. (B) The relative expression levels of PDS in the wheat spikes inoculated with BSMV:PDS at 2, 4, 8, 13, 22 and 30 dpi are determined by quantitative real-time PCR (qRT-PCR) (normalization to actin) compared the BSMV:00 control inoculated with BSMV:PDS. Each column represents the mean of three samples, and error bars indicate the standard deviation.

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