Figure 1

Schematic organization of the Barley stripe mosaic virus (BSMV) genomes and the inserts used for BSMV virus-induced-gene-silencing (VIGS). (A) Genomic organization of the three BSMV components α, β and γ drawn to scale of 300 bp. Open reading frames are indicated by boxes. The TA cloning site (TACS) designed for direct cloning of PCR products is positioned after the stop codon of the γb gene. (B) Schematic representation of full-length phytoene desaturase gene (PDS) and 1Bx14 cDNAs (black line) drawn to the same scale as above. The 185-bp fragment of PDS and the 176-bp fragment of 1Bx14 cDNAs indicated by boxes are inserts for BSMV-VIGS. P1 and P2, P6 and P7 are primer sets for generating the VIGS construct BSMV:PDS and BSMV:1Bx14, respectively. P3 and P4, P5 and P7 are primer sets for quantitative real-time RT-PCR (qRT-PCR) analysis of PDS and 1Bx14 transcripts, respectively. (C) Schematic representation of a 184-bp insert fragment of artificially synthesized sequence (asHMW) with one 85-bp sequence that is identical to most x-type glutenin subunit genes (asX) and one 99-bp sequence that is identical to most y-type genes (asY), drawn to the scale of 30 bp.