Figure 5

Expression of DHN s in V. vinifera during seed development. Total RNA was isolated from floral buds harvested 6 days prior to flower opening (−6 daf), flowers were harvested on the day of flower opening (0 daf), young berries were harvested 6 daf, and seeds were harvested from 12–66 daf. Transcript levels of DHN1, DHN2, DHN3, DHN4 normalized to the levels of the internal control, actin1, were determined using real-time qRT-PCR analysis. Each block represents the mean relative fold-change compared to baseline levels of expression from three experiments, while bars indicate standard deviations. In the case of DHN1, DHN2 and DHN4, baseline expression levels were those measured at −6 daf. In the case of DHN3, baseline expression was set to that measured at 18 daf as this was the first time point at which expression was noted. Times indicating flowering and veraison are indicated by arrows.