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Table 3 Primers used in this study

From: Characterization of resistance to pine wood nematode infection in Pinus thunbergiiusing suppression subtractive hybridization

Putative gene function Forward primer Reverse primer Fragment length (bp) GenBank Acc.
PR-1b family 5' -TGCCCCTTCAGGTAAATCGT-3' 5' -GCGGGTCGTAGTTGCAGATAA-3' 125 FY841927
PR-2 family (Beta-1,3-glucanase) 5' -CGACAACATTCGCCCCTTCT-3' 5' -CTGCAGCGCGGTTTGAATAT-3' 130 FY843702
PR-3 family (class I chitinase) 5' -ACCTACAGCGCCTTCATTGC-3' 5' -TGTGGTTTCATGCGACGTTT-3' 120 FY841849
PR-3 family (class Iv chitinase) 5' -CCATCGAAGCCCAGGTAATTT-3' 5' -AGCCGGGAAGCAATATTATGGT-3' 90 FY843470
PR-4 family 5' -CCCCGTTACTGTCAATTGCAT-3' 5' -AAAGCGTGACGGTGCGTATT-3' 90 FY841704
PR-5 family (thaumatin-like) 5' -GAACCAGTGCCCATACACAGTCT-3' 5' -CCTGCGGCAACGTTAAAAGTC-3' 96 FY842709
PR-6 family (proteinase inhibitor) 5' -TGCTGGCGGCATCTATTTTA-3' 5' -TAACACCTGCGCAAATGCA-3' 90 FY843534
PR-9 family (peroxidase) 5' -ACACCACCGTGCTGGACATT-3' 5' -GTGCGGGAGTCGGTGTAGAG-3' 118 FY842918
PR-10 family (ribonuclease-like) 5' -TGTCTCAAGTGGAGGCAAGGA-3' 5' -AAGCGACAATTTCAGGCAAAAC-3' 90 FY842956
Antimicrobial peptide 5' -GCGTTGCTCATACCCGTTTT-3' 5' -GCAGCACTTAGCACTGGATGAA-3' 90 FY841562
Cytochrome P450 5' -AACATGTCCTGCAGCACGAA-3' 5' -GTGCACCGCAAGTAAACCAA-3' 95 FY841345
Extensin 5' -CGAATGTAATTCCGAAGTTGCA-3' 5' -CCATCCCAAACCACCAGTCT-3' 110 FY844277
Heat shock protein 70 5' -AACACCACCATTCCCACCAA-3' 5' -CGAATTTGCCGAGCAGGTTA-3' 130 FY841300
Hydroxyproline-rich glycoprotein precursor 5' -GAGAAACTGGCACCGTCTTAGGA-3' 5' -ACCTCCCCCTCCATCTCACA-3' 140 FY843962
Metallothionein-like protein 5' -TCAGGCTGCTGCGTTATTTG-3' 5' -TGTCAGCGCAGTCACAATTTG-3' 120 FY842178
xyloglucan endotransglycosylase 5' -TCTGCGCCCCTACTTTTCC-3' 5' -AGCTGGGCGATTGATCATGT-3' 121 FY842425
Elongation factor-1 alpha 5' -GGGAAGCCACCCAAAGTTTT-3' 5' -TACATGGGAAGACGCCGAAT-3' 160 FY842441
  1. The putative functional genes from (a) to (h) were clearly discernible ESTs in susceptible libraries. The putative functional genes from (i) to (p) were clearly discernible ESTs in resistant trees. Elongation factor 1-alpha (EF1a) was used as the reference gene, and the data were calibrated relative to the transcript levels in resistant trees prior to nematode infection (at 0 dpi). The data are presented as the mean ± S.D. of three replicates. Means designed by the same letter did not significantly differ at P < 0.05 according to Tukey's HSD test