Figure 4

Expression levels of HMGR genes and sesquiterpenoid accumulation responses to JA signaling pathway. (A) Expression levels of EF1α and HMGR genes in response to JA determined by real-time qPCR and semi-qPCR analysis. Thirty-day-old plantlets of Atractylodes lancea were incubated with 5-mm mycelia disks; 0.01, 0.1, or 1 mmol L^-1 JA; or 1 mmol L^-1 IBU and harvested 18 d later for total RNA extraction and PCR analysis. Values are means ± SE (n = 3). Asterisks indicate significant differences (t-test; *, P <0.05; **, P <0.01). (B) Effects of JA on sesquiterpenoid accumulation. Plantlets were harvested after 18 d and evaluated for atractylone, β-eudesmol, and hinesol content. Values are means ± SE (n = 3). Bars with different lower-case letters were significantly different (one-way ANOVA, Duncan’s multiple range test, P <0.05).