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Table 3 Quantification of the efficiency of hardening and plant growth till flowering of haploid and doubled-haploid plants regenerated from microspore culture

From: A new microspore embryogenesis system under low temperature which mimics zygotic embryogenesis initials, expresses auxin and efficiently regenerates doubled-haploid plants in Brassica napus

Ploidy of plantlets

Hardening

Plant growth till flowering

Total number of plants#

Percentage of survival$

Total number of plants#

Percentage of survival$

Spontaneous DH

51

96.1 ± 3.8a

49

98.0 ± 2.0a

Haploid

35

91.6 ± 1.6b

33

96.4 ± 3.5a

  1. #9-26 plants tested in two replicates.
  2. $ Percentage: mean ± SEm.
  3. Means represented by the same small case letter are not significantly different as per two tailed t-test assuming unequal variance at α = 0.05 for survival during hardening or plantlet growth till flowering.