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Figure 5 | BMC Plant Biology

Figure 5

From: A new microspore embryogenesis system under low temperature which mimics zygotic embryogenesis initials, expresses auxin and efficiently regenerates doubled-haploid plants in Brassica napus

Figure 5

Early stages of the microspore embryogenesis pathway without suspensor induced at 18°C. Micrographs of phase contrast and DAPI epiflorescence to visualize the nuclei. a) Vacuolated microspore immediately after isolation, b) Two cell pro-embryo after 13-15d, c-f) Multicellular pro-embryos surrounded by the exine which appeared intact or just broken, displaying increasing number of cells (4–10 cells), and observed after 17-19d.

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BMC Plant Biology

ISSN: 1471-2229

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