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Figure 4 | BMC Plant Biology

Figure 4

From: A new microspore embryogenesis system under low temperature which mimics zygotic embryogenesis initials, expresses auxin and efficiently regenerates doubled-haploid plants in Brassica napus

Figure 4

Different structures and embryogenesis pathways in microspore cultures at 18°C. Panoramic views of cultures at 13 days (a) and 17 days (b, c) showing different structures and embryogenesis pathways at the same time points: small dead cells (thin arrows), rounded multicellular structures surrounded by exine (thick arrows) and uniseriate structures (open arrows) of two-three cells at 13 days (a) and longer structures at 17 days (b, c). b, c) Phase contrast (b) and DAPI epifluorescence (c) views of the same microscopic field.

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