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Figure 3 | BMC Plant Biology

Figure 3

From: A new microspore embryogenesis system under low temperature which mimics zygotic embryogenesis initials, expresses auxin and efficiently regenerates doubled-haploid plants in Brassica napus

Figure 3

Gametophytic- like developmental pathway in microspore cultures at 18°C. Mature pollen-like structures (arrows) observed after 6-8d. a) Phase contrast micrograph at low magnification, b) High magnification micrograph of mature pollen-like cells observed under merge of phase contrast and DAPI epiflorescence, showing the two small sperm-like nuclei and the larger vegetative-like nucleus. c) Evan’s blue staining for detection of cell death showing the mature pollen-like structures unstained and the smaller microspores stained (arrowhead).

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BMC Plant Biology

ISSN: 1471-2229

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