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Figure 2 | BMC Plant Biology

Figure 2

From: A new microspore embryogenesis system under low temperature which mimics zygotic embryogenesis initials, expresses auxin and efficiently regenerates doubled-haploid plants in Brassica napus

Figure 2

Microspore embryo production from two donor plants growth conditions and two inductive temperature treatments in vitro. A) Embryos induced by 32 ± 1°C treatment, culture at 30 days : (A1) embryos obtained from donors grown in growth chamber (15°C day/10°C night, 16 h photoperiod), and (A2) embryos obtained from donor plants grown in greenhouse (18°C with ambient light conditions). B) Embryos induced under 18 ± 1°C treatment, culture at 40 days: (B1) embryos obtained from donors grown in growth chamber, and (B2) embryos obtained from donor plants grown in greenhouse. (A3) and (B3): High magnification pictures showing the similar anatomy and different size of mature microspore embryos produced at 32 ± 1°C (A3, smaller embryos) and 18 ± 1°C (B3, larger embryos).

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BMC Plant Biology

ISSN: 1471-2229

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