Figure 11

Conversion of microspore derived embryos to plants. a, b, c) Consecutive stages during embryo germination; a) Slow air-dried embryos cultured at 18 ± 1°C which show distinct bipolar zygotic embryo-like germination in terms of simultaneous development of radicle and plumule end (indicated by red arrows) within 20d of incubation, b) b) Activation of chlorophyll and green pigmentation of cotyledons/embryo axis as a response to exposure to light (10-12d) after 20d dark incubation at 18±1°C. White arrow indicates a non-responsive/dead embryo, c) 2–3 leaf growth stage plantlets obtained after 21-28d culture at 25 ± 1°C, 16 h photoperiod. d, e, f, g) Consecutive stages during plantlet growth and transfer to photoautotrophic conditions; d) Plantlet elongation and development on MS medium, e) Transfer of plantlets to agropeat in glass jars for hardening, f) Regenerated plant transferred to greenhouse originated from plantlets at the vegetative growth stage (6–9 leafs), g) Flowering and further growth of a mature spontaneous DH plant. Arrow indicates bags placed for self-pollination.