Figure 10From: A new microspore embryogenesis system under low temperature which mimics zygotic embryogenesis initials, expresses auxin and efficiently regenerates doubled-haploid plants in Brassica napusLate stages of the microspore embryogenesis induced at 18°C: globular, and heart-torpedo embryos. a-d) Globular embryos with long suspensor like structures (26-30d) e, f) Heart-torpedo shaped embryo with suspensor-like structures (28-30d). Micrographs from squash preparations, the same embryo was observed under phase contrast (a, c, e), and DAPI epiflorescence for visualization of the nuclei (b, d, f).Back to article page