Figure 1

Quantification of embryo formation in microspore cultures from two donor plants grown conditions and three temperature treatments in vitro. The first three columns correspond to donor plants of growth chambers under 15°C day/10°C night, 16 h photoperiod. The last three columns correspond to donor plants from greenhouse grown under 18°C with ambient light conditions. Each histogram column represents the average number of microspore derived embryos obtained from three replicates wherein each replicate was defined by a minimum of two isolations and cultures. The total number of Petri dishes (n) observed were: 124 (1^st column), 119 (2^nd column), 123 (3^rd column), 132 (4^th column), 135 (5^th column) and 121 (6^th column). Histogram columns represented by different letters are significantly different from each other as per LSD_α=0.05 and bars indicate SEm. Absence of error bars for 25 ± 1°C indicates no response