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Figure 9 | BMC Plant Biology

Figure 9

From: The cytological changes of tobacco zygote and proembryo cells induced by beta-glucosyl Yariv reagent suggest the involvement of arabinogalactan proteins in cell division and cell plate formation

Figure 9

Distribution of endoplasmic reticulum and mitochondria in tobacco zygotes and proembryo cells. (A 1 -G 1 ) The fluorescence images of endoplasmic reticulum in zygotes and proembryo stained by DiOC6(3) under a confocal laser scanning microscope. (A 1 -D 1 )The green fluorescence signals of DiOC6(3) appear equably in the isolated in vivo zygotes and proembryo cells. (E 1 and F 1 ) The patterm of fluorescence signals of DiOC6(3) were disorganized in the zygote and proembryo cells treated with 50 μM β-GlcY reagent. (G 1 ) The fluorescence of DiOC6(3) in the untreated zygotes and proembryos cultured in vitro is similar to those in vivo. (H 1 -N 1 ) The fluorescence images of mitochondria in zygotes and proembryo stained by Rhodamine123 under a confocal laser scanning microscope. (H1-K1) The green fluorescence of Rhodamine123 in isolated zygotes and proembryos indicates mitochondria localization around nuclei. (L 1 -M 1 ) With 50 μM β-GlcY treatment, the distribution of mitochondria in proembryos is disordered. (N 1 ) The fluorescence of untreated samples cultured in vitro is similar to those in vivo. The nuclei are stained by DAPI and display blue fluorescence in A 1 -N 1 . (A 2 -N 2 ) The bright-field images of zygotes and proembryos corresponding to Figure A1-N1. Bars = 10 μm.

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